The histology core is excited to announce a new service for automated immunohistochemistry.
We have established protocols for the following markers:  

  • Ki 67
  • CD31
  • Pan Cytokeratin
  • Paralbumin
  • Phospho-STAT3
  • Samatostain.

Other markers may be developed upon request, as long as an appropriate antibody is available. 

For optimization of all other antibodies contact our veterinary pathologist:

Dr. Jean-Martin Lapointe   
Tel (514) 398-4400 ext. 00535    

Validated IHC Stains
Marker Target Population Target species Notes
ATP6IP2 (aka PRR) Cells expressing ATP6IP2 Mouse, human Specificity of staining uncertain
Beta-catenin cells expressing beta-catenin Mouse, rat, human, primate  
CEACAM-1 cells expressing CEACAM-1 Mouse, human Mouse monoclonal antibody
CD3 T lymphocytes Mouse, human, rat  
CD31 Endothelial cells Mouse only  
CD31 Endothelial cells Rat only  
CD34 Mesenchymal and hematopoietic stem cells, endothelium Human  
CD34 Mesenchymal and hematopoietic stem cells, endothelium Mouse, rat, others  
CD79a B lymphocytes all except mouse  
Cyclin D1 cells in proliferative phase mouse, human, rat  
CC3 (cleaved caspase-3) Apoptotic cells Mouse, human, rat  
GFAP (glial fibrillary acidic protein) Astrocytes All  
phospho-Histone H2A.X Cells expressing phosphorylation of Histone 2A.X at ser139 (indicating DNA damage) Mouse, human, rat May also stain cells undergoing division
phospho-Histone H3 Cells in mitotic phase Mouse, human  
Iba-1 Macrophages Mouse, human, rat, primate  
Ki-67 Proliferating cells (all cell cycle phases) Mouse, human, others  
Melanoma gp100 Melanoma cells Mouse, human used with red chromogen (AEC) to distinguish from melanin
MBP (myelin basic protein) Myelin (cerebral white matter, nerves) All  
NoGo-A Oligodendrocytes Mouse, rat, human, primate Cytoplasmic marker
Olig-2 Oligodendrocytes Mouse, rat, human, primate Nuclear marker
p53 Cells overexpressing p53 Mouse, rat Should recognize wild and mutant p53
pan-cytokeratin (AE1/AE3) Most epithelia (cytokeratins 1,2,3,4,5,6,7,10,14,15,16,19) all except mouse Does not react with cytokeratin 18 (most hepatocellular carcinomas negative). May react with glial cells and tumors
Parvalbumin Parvalbumin-positive neurons Human  
phospho-SMAD3 (ser423/425) Cells expressing SMAD3 phosphorylation at ser423/425 Mouse, human, primate  
phospho-STAT (tyr405) Cells expressing STAT3 phosphorylation at tyr705 Mouse, human, primate Staining highly dependent on tissue fixation conditions
Somatostatin Cells producing somatostatin Human  
Spondin 2 Cells expressing spondin 2 Mouse, human Specificity of staining uncertain
UCP-1 Cells expressing UCP-1 Mouse, rat, others Specificity of staining uncertain
vWF (vonWillebrand factor) endothelial cells, platelets Rat  
In preparation